| 阳和汤对骨关节炎软骨细胞HIF-1α mRNA影响的实验研究 |
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投稿时间：2007-10-30
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| 期刊信息：《中国骨伤》2008年，第21卷，第6期，第432-434页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:上海市重点学科建设项目(编号：T0303) |
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中文摘要:
目的：通过检测阳和汤对骨关节炎(OA)软骨细胞缺氧诱导因子-1α mRNA(HIF-1α mRNA)表达的影响，探讨阳和汤改善OA软骨退行性变的机制。
方法：30只新西兰大白兔随机分为正常组、模型组、阳和汤组，每组10只，按照Hulth法建立的膝骨关节炎模型，分别对关节软骨病理切片后采用Safranin O染色，光镜下观察，进行Mankin评分，同时实时荧光定量PCR检测HIF-1α mRNA在各组的表达。
结果：正常对照组Safranin O染色的Mankin评分与模型组差异有统计学意义(P=0.005),阳和汤组Safranin O染色减少或缺失程度较模型组轻，差异有统计学意义(P=0.003).模型组HIF-1α mRNA表达较正常组明显高，差异有统计学意义(P=0.035),阳和汤组HIF-1α mRNA表达明显低于模型组，差异有统计学意义(P=0.039).
结论：阳和汤可延缓关节软骨退行性变，其作用可能是通过调控HIF-1α mRNA的作用。 |
| 【关键词】骨关节炎 中药疗法 荧光光度测定法 HIF-1α mRNA |
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| Experiment research on the influence of Yanghe decoction(阳和汤)on the expression of HIF-1α mRNA in osteoarthritis |
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ABSTRACT
Objective: To explore the influence of Yanghe decoction(阳和汤)on the expression of HIF-1α mRNA in osteoarthritis(OA),so as to study mechanisms of Yanghe decoction(阳和汤)in the improvement of cartilage degeneration.
Methods: Thirty New Zealand white rabbits were randomly divided into 3 groups:control group(10 rabbits),OA model group(10 rabbits) and Yanghe decoction(阳和汤)treatment group(10 rabbits). The OA models were established by using Hulth method. The sections were stained with Safranin O for histological examination. Each sample was evaluated the cartilages histological characteristics according to the method of Mankin. Fluorescent quantitative PCR was used to detect the expression of HIF-1α mRNA in each group.
Results: There were significant differences in the Mankin score between the control group and the OA model group(P=0.005),either between the OA model group and the Yanghe decoction(阳和汤)treatment group(P=0.003). Fluorescent quantitative PCR indicated that HIF-1α mRNA significantly increased in the OA model group compared to that of the control group(P=0.035),but that expression was much lower in Yanghe decoction(阳和汤)treatment group compared to that of the OA model group(P=0.039).
Conclusion: The expression of HIF-1α mRNA plays an important role in OA. Yanghe decoction(阳和汤)is effective to protect the articular cartilage,which is achieved through regulating the expression of HIF-1α mRNA. |
| KEY WORDS Osteoarithritis Treatment with Chinese herbs Fluorophotomerty HIF-1α mRNA |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈朝蔚,陈永强.阳和汤对骨关节炎软骨细胞HIF-1α mRNA影响的实验研究[J].中国骨伤,2008,21(6):432~434 |
| 英文格式: | CHEN Chao-wei,CHEN Yong-qiang.Experiment research on the influence of Yanghe decoction(阳和汤)on the expression of HIF-1α mRNA in osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(6):432~434 |
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