| 骨肉瘤多药耐药表型的分化特性及矛盾相关性分析 |
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投稿时间：2007-07-26
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| 作者 | Author | 单位 | Address | E-Mail |
| 曾恒 |
ZENG Heng |
华中科技大学同济医学院附属同济医院矫形外科，湖北 武汉 430030 |
Department of Orthopaedics,Tongji Hospital Affiliated of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China |
zengheng@hotmail.com |
| 陈安民 |
CHEN An-min |
华中科技大学同济医学院附属同济医院矫形外科，湖北 武汉 430030 |
Department of Orthopaedics,Tongji Hospital Affiliated of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China |
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| 李锋 |
LI Feng |
华中科技大学同济医学院附属同济医院矫形外科，湖北 武汉 430030 |
Department of Orthopaedics,Tongji Hospital Affiliated of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China |
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| 杨彩虹 |
YANG Cai-hong |
华中科技大学同济医学院附属同济医院矫形外科，湖北 武汉 430030 |
Department of Orthopaedics,Tongji Hospital Affiliated of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China |
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| 期刊信息：《中国骨伤》2008年，第21卷，第5期，第368-372页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:科技部973课题子课题 (编号：2002CB513107) |
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中文摘要:
目的：探讨肿瘤的多药耐药性和恶性程度的关系，主要研究骨肉瘤细胞在向耐药表型的转化过程中，是否出现如骨源性干细胞在成熟分化过程中相似的生物学行为，或者是更复杂的生物学表型。
方法：采用不同表达程度P-gp的骨肉瘤耐药亚型细胞，并针对增殖、分化及转移关键性基因Stathmin及VEGF,应用原位杂交和RT-PCR来衡量Mdr1同Stathmin、VEGF表达的相关性。
结果：Mdr1/P170表型的骨肉瘤细胞的碱性磷酸酶的活性明显高于亲本细胞，说明Mdr表型的骨肉瘤细胞出现了分化。INH和RT-PCR结果揭示Mdr1 mRNA同VEGF、Stathmin mRNA表达呈负相关。
结论：Mdr1/P170的表达预示着骨肉瘤细胞向成熟状态分化，为研究Mdr及其逆转开拓了新的思路，为正确认识P-gp的功能提供了新的研究方法。 |
| 【关键词】骨肉瘤 多药耐药相关蛋白质类 血管内皮生长因子 |
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| Analysis of differentiation characters in osteosarcoma Mdr sublines |
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ABSTRACT
Objective: To explore the relationship between multidrug resistance(Mdr)and malignancy. To observe wheth- er P-glycoprotein(P-gp)overexpression had the same bioactivity as osteogenic stem cell turning into more mature cell or more complex phenotype when parent cell line turned to Mdr phenotype.
Methods: Six cell sublines of Mdr phenotype with different expression degree were analyzed. Stathmin generally identified in malignancy cell and stem cell,was a microtubule associated protein and the signal of differentiation in osteogenic stem cell. RT-PCR and hybridization in situ were used to analyze the relationship between Mdr1 mRNA and expression of Stathmin mRNA and VEGF mRNA.
Results: Morphological and functional analysis of Mdr phenotype showed the P-gp-positive cell lines were more differentiated than the parent cells in terms of enhanced activity of cellular alkaline phosphatase. These subclones all displayed a decrease in potential malignancy such as tumor growth rate and metastatic ability. A significant negative correlation could be identified between Mdr1 mRNA and expression of VEGF and Stathmin mRNA.
Conclusion: Expression of Mdr1/P170 indicated osteosarcoma cells differentiated towards more mature state,which will develop the new research field of Mdr and supply the new research method of the function of P-gp. |
| KEY WORDS Osteosarcoma Multidrug resistance-associataed proteins Vascular endothelial growth factor(VEGF) |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 曾恒,陈安民,李锋,杨彩虹.骨肉瘤多药耐药表型的分化特性及矛盾相关性分析[J].中国骨伤,2008,21(5):368~372 |
| 英文格式: | ZENG Heng,CHEN An-min,LI Feng,YANG Cai-hong.Analysis of differentiation characters in osteosarcoma Mdr sublines[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(5):368~372 |
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