| 药物干预对去卵巢大鼠关节软骨代谢的影响 |
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摘要点击次数: 2094
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投稿时间：2007-12-05
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| 期刊信息：《中国骨伤》2008年，第21卷，第3期，第196-199页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的：观察仙灵骨葆、戊酸雌二醇片、盐酸雷洛昔芬片和硫酸氨基葡萄糖胶囊对去卵巢大鼠关节软骨代谢标志物的影响及软骨病理改变，为临床防治妇女绝经后骨性关节炎的药物选择提供理论依据。
方法：实验采用雌性去卵巢SD大鼠，分别给予仙灵骨葆、雌激素、雌激素受体调节剂、硫酸氨基葡萄糖干预，并设假手术组。收取初始及去势后3、5、7周尿液，酶联免疫吸附法测定尿中II型胶原C端肽含量。11周后处死取膝关节做病理切片观测关节软骨面病变情况。
结果：大鼠去势后3、5周尿中CTX-II含量与去势前比较差异有统计学意义(P<0.05),3、5周时雌激素组、雌激素受体调节剂组，与模型组CTX-II含量比较差异有统计学意义(P<0.01),仙灵骨葆组与模型组比较差异有统计学意义(P<0.05),硫酸氨基葡萄糖组与模型组比较差异无统计学意义(P>0.05).病理学观察显示假手术组及各药物干预组关节软骨面病损程度与模型组比较差异有统计学意义(P<0.05).
结论：大鼠去势后尿中CTX-II含量明显增加(P<0.01),予雌激素替代疗法、雌激素受体调节剂，可以显着降低去势后CTX-II的代谢峰(P<0.01),有雌激素样作用的中成药仙灵骨葆同样有此作用(P<0.05),而硫酸氨基葡萄糖则无此作用。以上药物均可缓解去势后大鼠关节软骨的退变。 |
| 【关键词】软骨，关节 骨关节炎 骨质疏松，绝经后 中药疗法 |
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| Study on change of metabolism of articular cartilage in ovariectomized rats in the intervention of different drugs |
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ABSTRACT
Objective: To study the influence of metabolism of articular cartilage in ovariectomized rats in the intervention of different drugs.
Methods: Sixty-six virgin female Sprague-Dawley rats were randomly stratified into six groups:sham operation(SHAM)group,ovariectomized(OVX)group,estrogen replacement therapy(ERT)group,estrogen receptor modulator(ERM)group,Xianling Gubao(仙灵骨葆，XLGB) group,and glucosamine sulfate(GS)group. One group subjected to standard sham operation and the remaining five groups were ovariectomized. The five ovariectomized groups received treatment either with the vehicle or different drugs given as an oral suspension in the vehicle. Urine samples were obtained at initial time and weeks 3,5 and 7 after ovariectomy. ELISA method was used to measure the concentration of collagen II degradation products(CTX-II )in the urine. At study termination,histological analysis of the knee joint was used to assess the pathological changes of the articular cartilage.
Results: Compared with the baseline,the concentration of CTX-II at weeks 3 and 5 after ovariectomy were obviously different(P<0.05). Compared with the OVX group,concentration of CTX-II in the ERT group and ERM group were significant different(P<0.01). XLGB group also was obviously different with the OVX group at the two points(P<0.05). And interestingly in the histological examination in the OVX group was different with all the other groups.
Conclusion: The study indicates that estrogen deficiency accelerates destructive turnover of the collagen II in articular cartilage. The ERT,ERM,XLGB can relieve the effect. And all the drugs can delay the cartilage degradation caused by that effect. |
| KEY WORDS Cartilage,articular Osteoarthritis Osteoporosis, postmenopausal Treatment with Chinese herbs |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 高峰,杜宁.药物干预对去卵巢大鼠关节软骨代谢的影响[J].中国骨伤,2008,21(3):196~199 |
| 英文格式: | GAO Feng,DU Ning.Study on change of metabolism of articular cartilage in ovariectomized rats in the intervention of different drugs[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(3):196~199 |
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