| 虾青素对成骨细胞羟自由基氧化损伤的影响 |
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投稿时间：2007-07-31
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| 期刊信息：《中国骨伤》2008年，第21卷，第3期，第187-189页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的：在H2O2胁迫下，研究虾青素增强成骨细胞抗自由基损伤的作用。
方法：用H2O2作用MC3T3-E1成骨细胞，建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、虾青素低剂量组(1×10-7 mol/L)、虾青素中剂量组(1×10-6 mol/L)和虾青素高剂量组(1×10-5 mol/L).测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase,SOD)活性、活性自由氧(reactive oxygen species,ROS)含量、脂质过氧化物(lipid oxygen,LPO)含量，同时利用荧光偏振法测定细胞膜流动性。
结果：模型组细胞活力、SOD活性、细胞膜流动性均比各剂量虾青素组显着降低(P﹤0.01),而ROS、LPO含量均比各剂量虾青素组显着升高(P﹤0.01).
结论：H2O2摄入会导致大鼠成骨细胞的氧化损伤，而虾青素可以预防或降低此类损伤对细胞的影响。 |
| 【关键词】成骨细胞 超氧化物歧化酶 虾青素 |
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| Effects of Astaxanthin on the damage of osteoblast induced by H2O2 |
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ABSTRACT
Objective: To investigate the effect of Astaxanthin on enhancing the function of anti-oxidative damage in osteoblast.
Methods: MC3T3-E1 osteoblasts were randomly divided into five groups,including control group,model group,Astaxanthin group[low-dose(1×10-7 mol/L),middle-dose(1×10-6 mol/L),high-dose(1×10-5 mol/L)],in which the activity of cells,activity of superoxide dismutase (SOD),the content of reactive oxygen species (ROS),lipid oxygen(LPO)and membrane fluidity were tested and compared.
Results: Compared with Astaxanthin groups,the activity of cells,SOD activity and membrane fluidity in the model group were significantly decreased(P﹤0.01). However,the contents of ROS and LPO were sig- nificantly raised(P﹤0.01).
Conclusion: H2O2 can cause oxidative damage of MC3T3-E1 osteoblasts,but Astaxanthin can prevent or decrease its influence. |
| KEY WORDS Osteoblasts Superoxide dismutase Astaxanthin |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 裴凌鹏,董福慧,惠伯棣.虾青素对成骨细胞羟自由基氧化损伤的影响[J].中国骨伤,2008,21(3):187~189 |
| 英文格式: | PEI Ling-peng,DONG Fu-hui,HUI Bo-di.Effects of Astaxanthin on the damage of osteoblast induced by H2O2[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(3):187~189 |
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