| 丹参对骨骼肌缺血再灌注损伤低氧诱导因子-1αmRNA表达和血液流变学的影响 |
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投稿时间：2006-09-26
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| 作者 | Author | 单位 | Address | E-Mail |
| 张俐 |
ZHANG Li |
福建中医学院骨伤系,福建福州350003 |
Department of Orthopaedics &
Traumatology,Fujian University of TCM,Fuzhou 350003,Fujian,China |
zhanglil626@yahoo.com |
| 鲁力 |
LU Li |
福建中医学院骨伤系,福建福州350003 |
Department of Orthopaedics &
Traumatology,Fujian University of TCM,Fuzhou 350003,Fujian,China |
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| 李楠 |
LI Nan |
福建中医学院骨伤系,福建福州350003 |
Department of Orthopaedics &
Traumatology,Fujian University of TCM,Fuzhou 350003,Fujian,China |
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| 陈伯仪 |
CHEN Bo-yi |
福建中医学院骨伤系,福建福州350003 |
Department of Orthopaedics &
Traumatology,Fujian University of TCM,Fuzhou 350003,Fujian,China |
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| 期刊信息：《中国骨伤》2007年，第20卷，第5期，第298-301页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然基金项目(30572401);福建省自然基金项目(C0510023);福建省引进高层次人才项目(1401) |
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中文摘要:
目的:检测骨骼肌缺血再灌注损伤时低氧诱导因子-1α(hypoxia-induciblefactor-1,HIF-1α)mRNA表达、红细胞聚集、变形指数和骨骼肌肿胀系数的变化,探讨丹参对骨骼肌缺血再灌注损伤氧环境和血液流变学的影响。
方法:采用大鼠左侧提睾肌主滋养动脉夹闭造模,66只SD大鼠随机抽取6只作为正常组,对照组和丹参组各30只,此两组造模后分别取10、20、40、60、90min5个时点进行观测。取双侧提睾肌称重,计算肿胀系数。造模侧提睾肌液氮速冻,用RT-PCR检测HIF-1αmRNA表达;由腹主动脉取血1ml计算红细胞聚集指数和变形指数,观测血液流变学的变化。
结果:缺血再灌注损伤后丹参组HIF-1αmRNA的表达在各时点均弱于对照组,与正常组相比,各时点的HIF-1αmRNA表达水平差异有显著性意义。红细胞变形指数在缺血再灌注过程中各时点及组间对比差异无显著性意义。与对照组相比,丹参组红细胞聚集指数10min时差异有显著性意义(P<0.05),但随后呈逐渐下降趋势。而对照组红细胞聚集指数则呈波动性上升趋势。丹参组各相应时点的肿胀系数低于对照组。
结论:丹参能改善骨骼肌缺血再灌注损伤时组织的缺氧状况,调整组织细胞能量缺乏状况,并能改善骨骼肌缺血再灌注损伤时红细胞聚集率,降低血黏度,促进血液循环,改善微循环,利于肿胀的消退,对缺血再灌注损伤起到积极的防治作用。 |
| 【关键词】丹参 低氧诱导因子-1α 血液流变学 缺血再灌注损伤 肌,骨骼 大鼠 |
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| The mRNA expression of hypoxia-inducible factor-1α and hemorheology of skeletal muscle during ischemia reperfusion via Danshen(丹参) |
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ABSTRACT
Objective: To detect the expression of hypoxia-inducible factor-1(HIF-1α) and erythrocyte aggregation and edema index,and to explore the effect of oxygen circumstance and hemorheology of skeletal muscle during ischemia reperfusion(I/R) via Danshen.
Methods: Under anesthesia,the left cremaster muscles of 66 rats weighing(250±30) g were isolated,opened,denervated and spreaded onto a transparent acrylic microscope stage.Thirty rats were administered subcutaneously 30 min prior to reperfusion via Danshen served as Danshen group and the rest were administered subcutaneously via water served as control group.Six rats with the left cremaster muscles were open served as normal control group.Then the muscle from the left side was preserved for further examination of the expressions of HIF-1α mRNA by reverse transcriptase-polymerase chain reaction(RT-PCR).The edema index of cremaster muscles,the red blood cell aggregation index and blood adherence were examined.
Results: Following 3 hours of ischemia,the expression of HIF-1α was decreased in Dashen group especially by the end of 40,60 and 90 min reperfusion.There was a significant difference between the ischemia side ane control side and the expression of HIF-1α mRNA was lower than that in control group.Following 3 hours of ischemia,the red blood cell aggregation index of Danshen group was increased at 10 min compared with the normal control group and decreased in the following time points.There was no significant difference by the end of 90 min.In control group,following 3 hours of ischemia it increased!and there was a significantly difference at 90 min.There was a significant difference of the edema index at 60 and 90 min.The edema index kept lower all time points in the Danshen group.
Conclusion: Danshen can regulate the condition of lacking of energy in tissues and cells by improving the hypoxic condition of skeletal muscle during ischemia reperfusion injury.It can also significantly increase red blood cell aggregation index,decrease blood adherence and edema index which indicates Danshen has potential clinical benefits during I/R. |
| KEY WORDS Danshen Hypoxia-inducible factor-1(HIF-1α) Hemorheology Ischemia reperfusion injury Muscle,skeletal Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张俐,鲁力,李楠,陈伯仪.丹参对骨骼肌缺血再灌注损伤低氧诱导因子-1αmRNA表达和血液流变学的影响[J].中国骨伤,2007,20(5):298~301 |
| 英文格式: | ZHANG Li,LU Li,LI Nan,CHEN Bo-yi.The mRNA expression of hypoxia-inducible factor-1α and hemorheology of skeletal muscle during ischemia reperfusion via Danshen(丹参)[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(5):298~301 |
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