| RANKL在佐剂性关节炎大鼠滑膜中的表达及亚砷酸对其影响 |
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投稿时间：2006-05-19
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| 作者 | Author | 单位 | Address | E-Mail |
| 李龙 |
LI Long |
贵阳医学院附属医院肾内科,贵州贵阳550004 |
Department of Nephrology,the Affiliated of Guiyang Medical College,Guiyang 550004,Guizhou,China |
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| 周忠启 |
ZHOU Zhong-qi |
临沂市人民医院 |
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| 曾家顺 |
ZENG Jia-shun |
贵阳医学院附属医院肾内科,贵州贵阳550004 |
Department of Nephrology,the Affiliated of Guiyang Medical College,Guiyang 550004,Guizhou,China |
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| 期刊信息：《中国骨伤》2007年，第20卷，第5期，第292-294页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:贵州省省长基金(编号:黔科技办2004.07) |
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中文摘要:
目的:研究细胞核因子-κB受体活化因子配基(receptoractivatorofnuclearfactor-κBligand,RANKL)在佐剂性关节炎大鼠发病中的作用,探讨亚砷酸对RANKL的影响及其对佐剂性关节炎大鼠的治疗作用。
方法:40只大鼠随机分成4组:正常对照组、模型组、低剂量亚砷酸组(LSA,1.5mg.kg-1.d-1)、高剂量亚砷酸组(HSA,3.0mg.kg-1.d-1),正常对照组与模型组给予生理盐水2ml/d。免疫组化、原位杂交方法检测各组RANKL蛋白及其mRNA的表达;HE染色观察各组滑膜组织形态变化。
结果:与正常对照组比较,模型组RANKL蛋白及其mRNA大量表达(P<0.01);与模型组比较,LSA组及HSA组RANKL蛋白及其mRNA表达降低(P<0.01),且HSA组更明显。
结论:RANKL在大鼠佐剂性关节炎发生发展中具有重要作用;亚砷酸能下调RANKL蛋白及其mRNA的表达,提示亚砷酸对RA有一定的治疗作用。 |
| 【关键词】核因子κB受体活化因子配基 亚砷酸盐类 关节炎,类风湿 大鼠 |
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| Expression of recept activator of nuclear factor-κB ligand (RANKL) of synovium in adjuvant arthritis rats and effects of sodium arsenite on them |
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ABSTRACT
Objective:To study the expression of receptor activator of nuclear factor-κB ligand(RANKL)in synovium of adjuvant arthritis rats and effects of sodium arsenite(SA)on RANKL.
Methods:Forty Wistar female rats were randomly divided into 4 groups:normal control group,model group,low concentration sodium arsenite group(LSA)and high concentration sodium arsenite group(HSA).LSA group and HAS group were treated with SA(1.5 mg.kg-1.d-1 and 3 mg kg-1.d-1)through abdominal injection,the normal control group and the model group were treated with saline(1 ml/d).The expressions of RANKL protein and mRNA of synovium by immunohistochemistry and by in situ hybridization.Light microscope was used to observe the synovium by HE.
Results:Compared with normal control group,the expressions of RANKL protein and mRNA in the synovium were up-regulated in the model group(P<0.01)and were inhibited by sodium arsenite(P<0.01),especially in the HAS group.
Conclusion:RANKL may play an important role in the development adjuvant arthritis.Sodium arsenite can down-regulate the expressions of RANKL protein and mRNA and may have some therapeutic effects in rheumatoid arthritis. |
| KEY WORDS Receptor activator of nuclear factor-κB ligand,RANKL Arsenites Arthritis,rheumatoid Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李龙,周忠启,曾家顺.RANKL在佐剂性关节炎大鼠滑膜中的表达及亚砷酸对其影响[J].中国骨伤,2007,20(5):292~294 |
| 英文格式: | LI Long,ZHOU Zhong-qi,ZENG Jia-shun.Expression of recept activator of nuclear factor-κB ligand (RANKL) of synovium in adjuvant arthritis rats and effects of sodium arsenite on them[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(5):292~294 |
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