| 膝骨关节炎滑膜细胞体外培养及生物学特性观察 |
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摘要点击次数: 1953
全文下载次数: 1790
投稿时间：2006-07-10
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| 期刊信息：《中国骨伤》2006年，第19卷，第11期，第656-658页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金资助项目(30472222) |
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中文摘要:
目的:观察原发性膝骨关节炎患者滑膜细胞的体外生长特性,探讨骨性关节炎的发病机制。
方法:组织块培养法分离早期与晚期(依据Kellgren和Lawrence的放射学诊断标准划分)OA患者滑膜细胞进行培养,用光镜、电镜、组织化学
方法:等观察鉴定该细胞类型。
结果:患者滑膜细胞符合巨噬样滑膜细胞(A型细胞)及成纤维细胞样滑膜细胞(B型细胞)的特征,早期与晚期滑膜细胞在生长周期及细胞超微结构等方面存在差异。晚期较早期滑膜细胞生长周期长、生长极向性差、细胞内溶酶体丰富。
结论:组织块培养法可以有效分离人类关节滑膜细胞,建立原发性膝骨关节炎患者滑膜细胞系。 |
| 【关键词】滑膜 细胞,培养的 骨关节炎,膝 |
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| Biologic characteristics of human synoviocytes of gonarthritis in vitro |
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ABSTRACT
Objective:To investigate the biological characteristics of human synoviocytes of primary gonarthritis in vitro.
Methods:Human synoviocytes were isolated from synovial membrane(earlier stage and later stage of OA) by tissue culture isolation,which were identified by light microscope,electro microscope,histochemical method.
Results:The isolated cells were in acordance with the characteristics of macrophage-like synoviocytes(type-A synoviocytes) and fibroblast-like synoviocytes (type-B synoviocytes).Differences showed in the growth cycles,differentiations and ultra microstructures between the two stages.
Conclusion:Tissue culture isolation is an effective isolation of human synoviocytes to establish the human synoviocyte line of primary gonarthritis. |
| KEY WORDS Synovial membrane Cells,cultured Osteoarthritis,knee |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张鹏,石关桐,郑昱新.膝骨关节炎滑膜细胞体外培养及生物学特性观察[J].中国骨伤,2006,19(11):656~658 |
| 英文格式: | ZHANG Peng,SHI Guan-tong,ZHENG Yu-xin.Biologic characteristics of human synoviocytes of gonarthritis in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2006,19(11):656~658 |
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