| 骨髓基质细胞与可降解明胶-聚羟基丁酸酯膜复合培养的生物合成功能分析 |
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投稿时间：2005-12-30
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| 期刊信息：《中国骨伤》2006年，第19卷，第7期，第413-416页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金青年项目(30200063) |
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中文摘要:
目的:从生物合成功能角度,探讨骨髓基质细胞(BMSCs)与可降解明胶-聚羟基丁酸酯(G-PHB)膜复合培养的生物相容性。
方法:以BMSCs分别与G-PHB、聚羟基丁酸酯(PHB)、明胶(G)膜材料复合培养,并设立空白对照组,各组通过3H-TdR法检测DNA合成;考马斯亮蓝法检测蛋白质合成;对硝基苯磷酸盐法(PNP)测定碱性磷酸酶(ALP)水平;放免法测定骨钙素(OCN)表达;流式细胞术测定纤维连接蛋白(FN)表达。
结果:G-PHB组DNA合成优于PHB组和空白对照组(P<0.05)。总蛋白合成与各对照组无显著性差异(P>0.05),但成骨特异性标志ALP、OCN以及细胞黏附指标FN均明显优于其他各组(P<0.05)。
结论:G-PHB材料对于种子细胞BMSCs黏附、增殖与成骨定向分化有明显的促进作用,是一种有效上调细胞生物合成功能的新型可降解材料。 |
| 【关键词】骨髓基质细胞 可降解的 生物功能 |
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| Analysis of biosynthetic function on mixed culture of BMSCs and absorbable G-PHB membrane |
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ABSTRACT
Objective:To investigate biological compatibility on mix culture of BMSCs and absorbable G-PHB membrane basing on standpoint of biosynthetic function.
Methods:BMSCs were cultured with G-PHB,PHB and Glutin（G） membrane respectively to establish the blank control group.In the groups,the DNA and protein synthesis,Akaline phosphatase（ALP） level,Osteocalcin（OCN） and fibronection（FN） expression of BMSCs were respectively detected by 3H-TdR,Coomassie brilliant blue staining,paranitro-pheneye phosphate（PNP）,radioimmunoassay（RIA）,flow cytometry（FCM）.
Results:The DNA synthesis in the G-PHB group was higher than that of the PHB and the blank control group（P<0.05）.There was no significant difference in total protein synthesis were observed G-PHB group and other groups（P>0.05）.The ALP level,OCN and FN expression in G-PHB group were better than those of groups（P<0.05）.
Conclusion:The G-PHB has obviously auxo-action for adhesiveness and proliferation of BMSCs,and osteogenesis oriented differentiation.It is a new pattern and absorbable material which can effective cause up-regulation of biosynthesis function of cell. |
| KEY WORDS Bone marrow stem cells Absorbable Biological function |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 易诚青,刘建湘,刘日光,李新春,郭晓东.骨髓基质细胞与可降解明胶-聚羟基丁酸酯膜复合培养的生物合成功能分析[J].中国骨伤,2006,19(7):413~416 |
| 英文格式: | YI Cheng-qing,LIU Jian-xiang,LIU Ri-guang,LI Xin-chun,GUO Xiao-dong.Analysis of biosynthetic function on mixed culture of BMSCs and absorbable G-PHB membrane[J].zhongguo gu shang / China J Orthop Trauma ,2006,19(7):413~416 |
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