| 人参皂甙Rb1对体外软骨细胞Ⅱ型胶原mRNA表达的影响 |
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投稿时间：2004-12-02
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| 作者 | Author | 单位 | Address | E-Mail |
| 王昭佩 |
WANG Zhao-pei |
广州中医药大学附属广东省中医院骨科,广东广州510407 |
Department of Orthopedics,the Second Affiliated TCM Hospital of Guangzhou Un iversity of TCM,Guangzhou 510407, Guangdong,China |
yrxtcm@Tom.com |
| 杨仁轩 |
YANG Re n-xuan |
广州中医药大学附属广东省中医院骨科,广东广州510407 |
Department of Orthopedics,the Second Affiliated TCM Hospital of Guangzhou Un iversity of TCM,Guangzhou 510407, Guangdong,China |
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| 许树柴 |
XU Shu-chai |
广州中医药大学附属广东省中医院骨科,广东广州510407 |
Department of Orthopedics,the Second Affiliated TCM Hospital of Guangzhou Un iversity of TCM,Guangzhou 510407, Guangdong,China |
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| 郭玉海 |
GUO Yu-hai |
广州中医药大学附属广东省中医院骨科,广东广州510407 |
Department of Orthopedics,the Second Affiliated TCM Hospital of Guangzhou Un iversity of TCM,Guangzhou 510407, Guangdong,China |
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| 邓晋丰 |
DENG Jin-f eng |
广州中医药大学附属广东省中医院骨科,广东广州510407 |
Department of Orthopedics,the Second Affiliated TCM Hospital of Guangzhou Un iversity of TCM,Guangzhou 510407, Guangdong,China |
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| 期刊信息：《中国骨伤》2005年，第18卷，第9期，第543-545页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:广东省中医药管理局资助项目(编号:102038) |
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中文摘要:
目的:探讨兔体外软骨细胞不同代系间Ⅱ型胶原mRNA表达的差异以及人参皂甙Rb1对第2代软骨细胞Ⅱ型胶原表达的影响。
方法:在成功分离培养软骨细胞后,以细胞爬片法检测原代及第2、4代软骨细胞Ⅱ型胶原mRNA表达的差异。以浓度为0.01μmol·L -1,0.1μmol·L -1,1μmol·L -1,10μmol·L -1,100μmol·L -1的Rb1作用于贴壁3d后的第2代病理软骨细胞,观察各浓度组软骨细胞Ⅱ型胶原mRNA表达情况。
结果:原代、第2代、第4代软骨细胞Ⅱ型胶原mRNA表达的强度依次下降,适宜浓度的Rb1可增强第2代软骨细胞Ⅱ型胶原的表达,维持软骨细胞的正常表型。
结论:兔软骨细胞不同代系间Ⅱ型胶原mRNA的表达存在一定差异,人参皂甙Rb1可能有利于第2代软骨细胞Ⅱ型胶原的表达。 |
| 【关键词】软骨细胞 基因表达 中草药 |
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| Effects of ginsenoside Rb1 on the expression of collagen Ⅱ mRNA of chondrocytes in vitro |
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ABSTRACT
Objective:To study collagen Ⅱ mRNA expression of chon drocy tes in differ ent generations and the effects of ginse noside Rb1 on the expression of collagen Ⅱ mRNA of the second generation chon drocytes in rabbits.
Methods:After the rabbits’chondrocytes were cultured successfully,the expressions of collagen Ⅱ mRNA of the first,the second and the third generation chondrocytes were detec ted.Different density ginsenoside Rb1,in cluding 0.01 μmol·L -1,01 μmol·L -1,1 μmol·L -1,10 μmol·L -1and 100 μmol·L -1,were exerted to the second generat ion chondrocyte,and then the expressions of collagen ⅡmRNA were observed.
Results:The e xpression intensity of collagen Ⅱ mRNA o f the first,the second and the third generati on chondrocytes decreased gradually.The ginsenoside Rb1 with suitable density could strength en the expression of collagen Ⅱ mRNA,and restrai n the expression of specific collagen Ⅱ .
Conclusion:T he expressions of collagen Ⅱ mRNA in dif ferent generation chondrocytes is different.Ginsenoside Rb 1 may be benefit to increase the express ion of collagen Ⅱ mRNA in the second generat ion chondrocytes. |
| KEY WORDS Chondrocytes Gene expression Drugs,Chinese herbal |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王昭佩,杨仁轩,许树柴,郭玉海,邓晋丰.人参皂甙Rb1对体外软骨细胞Ⅱ型胶原mRNA表达的影响[J].中国骨伤,2005,18(9):543~545 |
| 英文格式: | WANG Zhao-pei,YANG Re n-xuan,XU Shu-chai,GUO Yu-hai,DENG Jin-f eng.Effects of ginsenoside Rb1 on the expression of collagen Ⅱ mRNA of chondrocytes in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(9):543~545 |
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