| 活血化瘀汤影响骨折愈合早期一氧化氮合酶活性的作用机制探讨 |
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投稿时间：2004-08-20
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| 期刊信息：《中国骨伤》2005年，第18卷，第2期，第93-96页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:教育部科学技术研究重点项目 (NO .0 30 63);福建省教育厅科学技术重点项目 (NO .JA0 2 2 2 8) |
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中文摘要:
目的:检测骨折愈合早期大鼠血清一氧化氮合酶(NOS)及其异构体的酶活性及活血化瘀汤对其的影响。
方法:选用雄性SD大鼠20只,建立胫骨中段标准骨折模型,随机分为两组,每组10只,分别以活血化瘀汤灌胃(中药组)或生理盐水灌胃(对照组);采用化学比色法测定骨折后1、4、7、14、21dNOS总量、诱导型一氧化氮合酶(iNOS)及原生型一氧化氮合酶(cNOS)的活性。
结果:骨折愈合早期血清cNOS酶活性增强,骨折后4d达峰值。血清iNOS酶活性于骨折后14d达峰值;中药组NOS酶总量维持在较高水平,cNOS酶活性于骨折后14d达到峰值。两组比较,骨折后7、14、21d血清NOS总量及骨折后14dcNOS酶活性提高非常显著(P<001),4dNOS总量、7dcNOS酶活性显著提高(P<005)。血清iNOS酶活性在骨折后4、7、21d中药组高于对照组,1、14d低于对照组,但无统计学意义。
结论:骨折愈合早期血清NOS酶活性随时间变化,不同的异构体变化不同;活血化瘀汤能激活大鼠骨折后血清NOS酶活性的作用,对不同的NOS同工酶在不同的时间产生的作用不同。提示活血化瘀汤可通过不同方式激活NOS酶活性促进骨折愈合。 |
| 【关键词】一氧化氮 一氧化氮合酶 骨折愈合 中草药 |
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| Enzymatic activities of nitric oxide synthase during fracture healing with Huoxue Huayu decoction(活血化瘀汤) |
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ABSTRACT
Objective: The enzymatic activities of nitric oxide synthase(NOS)were examined for effects of the Huoxue Huayu decoction(活血化瘀汤)during rat fracture healing.
Methods: Standardized closed fracture of the left tibia in isoflurane anesthetized Sprague Dawley rats was induced using a modified technique.All rats were filled with Huoxue Huayu decoction served as treatment or distilled water served as controls after fractures.Blood serum was determined by the enzymatic activity of NOS,inducible NOS(iNOS) and constitutive NOS(cNOS) on the 1st,4th,7th,14th and 21st day after fracture.
Results: It increased gradually on the enzymatic activity of cNOS on the 4st day and iNOS on the 14th day but there was no significant difference during early fracture healing.After treatment of Huoxue Huayu decoctin,the enzymatic activity of NOS kept higher level from the 4th day during fracture healing especially the 7th,14th and 21st day( P <0 01).The enzymatic activity of cNOS increased significantly on the 7th( P <0 05) and 14th day after fracture( P <0 01).
Conclusion: The enzymatic activity of NOS and its isomerase are different during early fracture healing.The enzymatic activity of NOS and its isomerase are more active after treatment of Huoxue Huayu decoction during observation time points.The study indicates the enzymatic activity of NOS has been improved after the treatment of Huoxue Huayu decoction during early fracture healing. |
| KEY WORDS Nitric oxideNitric oxide synthaseFracture healingDrugs,Chinese herbal |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张俐,周海兵,刘烈刚.活血化瘀汤影响骨折愈合早期一氧化氮合酶活性的作用机制探讨[J].中国骨伤,2005,18(2):93~96 |
| 英文格式: | ZHANG Li ,ZHOU Hai-bing,LIU Lie-gang. .Enzymatic activities of nitric oxide synthase during fracture healing with Huoxue Huayu decoction(活血化瘀汤)[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(2):93~96 |
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