| 健骨颗粒对骨质疏松模型大鼠垂体-肾上腺轴的影响 |
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投稿时间：2003-07-04
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| 期刊信息：《中国骨伤》2004年，第17卷，第1期，第19-21页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:福建省自然科学基金重大项目(C9830001);教育部骨干教育资助项目(No:1946) |
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中文摘要:
目的:从垂体-肾上腺轴角度探讨健骨颗粒治疗Ⅰ型原发性骨质疏松症的作用机制。
方法:通过切除SD大鼠双侧卵巢建立Ⅰ型原发性骨质疏松模型,随机分成3组,分别喂服健骨颗粒、骨松宝和生理盐水。用药12周后处死取材,检测血清皮质醇(COR)浓度,取脑垂体行ACTH细胞免疫化学染色(S-P法)并形态计量,肾上腺行HE染色。
结果:切除卵巢12周后大鼠出现骨质疏松,血清COR浓度以及垂体ACTH阳性细胞数和染色面积均明显高于假手术组(行腹部切口,但不切除卵巢)。用药12周后健骨颗粒组血清COR水平、垂体ACTH阳性细胞数和染色面积均较生理盐水组降低或减少。
结论:补肾健脾中药健骨颗粒可有效调节去卵巢骨质疏松模型大鼠垂体-肾上腺轴的病理变化和功能状态,抑制雌激素水平降低后的异常骨吸收。 |
| 【关键词】骨质疏松,绝经后 垂体 肾上腺 中草药 |
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| Effect of Jiangu granule on the pituitary-adrenal gland axis of ovariectomized rats |
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ABSTRACT
Objective:To investigate the effect of Jiangu granule on the primary osteoporosis(POP) in the view of the pituitary-adrenal gland axis.
Methods:POP animal model type I was made by ovariectomized in SD rats,Which were randomly divided into Jiangu granule group,Gusongbao group and saline group.The animals were killed at 12 weeks after treatment.The levels of serum COR were measured.The ACTH cells in pituitary were stained with immunocytochemical method and observed with morphometry.The adrenal gland was stained with HE.
Results:Osteoporosis occurred at 12 weeks after ovariectomy.The level of the serum COR and the number of the positive ACTH cells were higher than sham-operation group.After 12 weeks of treatment,the levels of the serum COR and the numbers of the positive ACTH cells in Jiangu granule groups were lower than the saline group.
Conclusion:Jiangu granule can adjust the functional statement of the pituitary-adrenal gland axis of ovariectomized rats by means of "invigorating the kidney and spleen"and inhibit the abnormal bone resorption after ovariectomy. |
| KEY WORDS Osteoporosis,postmenopausal Pituitary gland Adrenal glands Drugs,Chinese herbal |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 林燕萍,周瑞祥,马建华,黄美雅.健骨颗粒对骨质疏松模型大鼠垂体-肾上腺轴的影响[J].中国骨伤,2004,17(1):19~21 |
| 英文格式: | LIN Yan-ping,ZHOU Rui-xiang,MA Jian-hua,HUANG Mei-ya.Effect of Jiangu granule on the pituitary-adrenal gland axis of ovariectomized rats[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(1):19~21 |
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