| OA患者关节液与血清中金属蛋白酶及其抑制剂的检测及临床意义 |
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投稿时间：2001-12-30
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| 期刊信息：《中国骨伤》2002年，第15卷，第10期，第580-583页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:卫生部科研基金资助课题(编号:96-2-254) |
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中文摘要:
目的:本文探讨基质金属蛋白酶(MMP-3、MMP-1)及组织抑制剂(TIMP-1)对骨关节炎发病作用机理研究。
方法:对46例膝关节骨性关节炎患者,20例正常人血清标本,以及8例正常人关节液标本中的MMPs,TIMP及细胞因子进行了研究。MMP-3、MMP-1、TIMP-1的检测均采用双抗体夹心ELISA法。
结果:OA患者血清中MMP-3、MMP-1的水平没有性别差异,患者关节液中的MMP-3含量男性显著高于女性,约为1～2.5倍。患者关节液中MMP-3、MMP-1、TIMP-1的含量均显著高于血清中的含量。患者血清中只有MMP-3的水平显著高于正常对照组,MMP-1、TIMP-1的水平与正常对照组无显著性差异;而关节液中MMP-3、MMP-1、TIMP-1的水平均显著高于正常对照组。本研究结果还显示MMP-3、MMP-1和TIMP-1与病程成正相关。
结论:OA患者关节液中MMP-3水平增高的幅度比TIMP-1要大,由于二者间的相对平衡被破坏而促进了关节软骨的降解,导致关节软骨进行性破坏。 |
| 【关键词】骨关节炎 金属蛋白酶类 病理过程 |
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| Serum and synovial fiuid levels of matrix metalloproteinases and its tissue inhibitor in patients with os-teoarthritis |
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ABSTRACT
Objective:To measure serum and synovial fluid levels of matrix metalloproteinases(MMP-3, MMP-1),and tissue inhibitor of metalloproteinases(TIMP-1) for the investigation of the mechanism of os-teoarthritis (OA)
Methods:46 patients with knee osteoarthritis were studied and 20 specimens of normal serum and 8 specimens of normal synovial fluid were used for the measurements. Serum and synovial fluid levels of MMPs(MMP-3,MMP-1) ,TIMP(TIMP-1) were measured by enzyme immunoassays.
Results:In the OA patients, the synovial fluid levels of MMP-3,MMP-1,TIMP-1 were both significantly higher than that in the serum,MMP-3 was approximately 200 times higher than in serum. Only the synovial fluid level of MMP-3 was higher in males than in females. The synovial fluid levels of MMP-1, MMP-3 ,TIMP-1 were elevated in OA when compared with normal, with only higher levels of MMP-3 in serum found in OA. The synovial fluid concentration of MMP-3,MMP-1 and TIMP-1 were related to the degree of OA.
Conclusion:These findings demonstrate that the balance between the synovial fluid level of MMP-3 and TIMP-1 is broken and might promote cartilage degradation. |
| KEY WORDS Osteoarthritis Metalloproteinases Pathologic processes |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李德达,张凯,王毅,于顺禄,李世民,刘长明,马淑香,王介民,赵春英.OA患者关节液与血清中金属蛋白酶及其抑制剂的检测及临床意义[J].中国骨伤,2002,15(10):580~583 |
| 英文格式: | LI De-da,ZHANG Kai,WANG Yi.Serum and synovial fiuid levels of matrix metalloproteinases and its tissue inhibitor in patients with os-teoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(10):580~583 |
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