| 大鼠脊髓损伤后腓肠肌GDNF基因表达及意义 |
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投稿时间：2000-07-23 修订日期：2000-10-13
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| 期刊信息：《中国骨伤》2001年，第14卷，第8期，第465-467页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金资助项目(39500044) |
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中文摘要:
目的：探讨大鼠脊髓损伤后,后肢肌GDNFmRNA表达的变化及其意义。
方法：选用SD雄性大鼠30只,根据脊髓损伤时间随机分为5组,即伤前、伤后3、7、10及21天组。采用Allens方法致大鼠脊髓T13段不完全性损伤,以βActin为内参照物,半定量RT-PCR方法,观察大鼠腓肠肌在脊髓损伤前、后不同时间GDNFmRNA表达。
结果：脊髓损伤前GDNFmRNA在大鼠腓肠肌微量表达,脊髓损伤后3天表达上调至3倍,7天上调至10倍,10天上调至4倍,21天仍高于正常水平。
结论：脊髓损伤后骨骼肌GDNFmRNA表达上调,可能是神经元通过”胞体-轴突-靶器官“途径的一种自我保护反应,用GDNF治疗脊髓损伤时应长期用药。 |
| 【关键词】脊髓损伤 基因表达调控 肌 |
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| GDNF gene expression and its significance on gastrocnemius mucle of rats with injuried spinal cord |
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ABSTRACT
Objective:To investigate the varieties and its significance of GDNF gene expression on hindlimbs of rats with crushed injury of the spinal cord (SCI)
Methods:30 male SD rats were divided randomly into 5 groups(pro operation,3th,7th,10th and 21st after operation) based on the time of spinal cord injury produced with Allens method.GDNFmRNA expression on gastrocnemius mucle of the rats with spinal cord injury at different times after the injury were observed using semi quantitaive RT PCR method with β Actin as a inner consult.
Results:Minimal expression of GDNFmRNA was found in gastrocnemius mucle in the normal rats,and it up regulated 3 times 3 days after SCI,10 times 7 days,4 times 10 days and slight higher than normal level 21 days after SCI.
Conclusion:It may be one kind of self protective reaction of skeletal muscle to express high standard GDNFmRNA through "motorneuron axon target" route in the early stage after SCI.GDNF should be supplied early and long term treatment of SCI. |
| KEY WORDS Spinal cord injuries Gene expression regulation Muscles |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 宋海涛,贾连顺,陈坚,陈哲宇,沈强,史建刚.大鼠脊髓损伤后腓肠肌GDNF基因表达及意义[J].中国骨伤,2001,14(8):465~467 |
| 英文格式: | SONG Hai tao,JIA Lian shun,CHEN Jian.GDNF gene expression and its significance on gastrocnemius mucle of rats with injuried spinal cord[J].zhongguo gu shang / China J Orthop Trauma ,2001,14(8):465~467 |
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